Sequence Detection Systems Quantitative Assay Design and Optimization
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چکیده
PE Biosystems has developed guidelines enabling streamlined design and implementation of real-time quantitative PCR assays. The use of these guidelines makes it easy to apply either the fluorogenic 5 ́ nuclease assay or SYBR Green I double-stranded DNA binding dye chemistry to any real-time quantitative PCR system. Specific assay design and optimization guidelines minimize the time and cost of assay implementation, while providing reliable and robust assay performance.
منابع مشابه
Sequence Detection Systems Quantitative Assay Design and Optimization
PE Biosystems has developed guidelines enabling streamlined design and implementation of real-time quantitative PCR assays. The use of these guidelines makes it easy to apply either the fluorogenic 5 ́ nuclease assay or SYBR Green I double-stranded DNA binding dye chemistry to any real-time quantitative PCR system. Specific assay design and optimization guidelines minimize the time and cost of a...
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